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Pen a 1, Tropomyosin, Shrimp

Pen a 1, Tropomyosin, Shrimp

Pen a 1, Tropomyosin, Shrimp Scientific Information
Type: Component
Name; WHO/IUIS: Pen a 1, Tropomyosin, Shrimp
Source Material: rPen a 1 is a CCD-free recombinant protein
Other Names: Tropomyosin
Allergen code: f351

Clinical Relevance

Shrimp are small invertebrate marine crustaceans with 10 jointed legs (decapod) on the thorax, well-developed swimmerets on the abdominal segments, and a body that is compressed laterally. They live on the floor of oceans and lakes. There are over 2,000 different species of Shrimp worldwide.

One common commercial Shrimp is of the genus Peneus.

There are several other crustacean forms that are commonly called Shrimp although they do not belong to the same order as the true Shrimp, order Decapoda (phylum Arthropoda, subphylum Crustacea, class Malacostraca), which also includes the Lobsters and Crabs.

Shrimp may be divided into 3 basic categories: cold-water or northern; warm-water, tropical, or southern; and freshwater. However the nomenclature is complicated and the term “Shrimp” sometimes applies to all crustaceans of the Natantia group, regardless of size. The terms “Prawn” and “Scampi” are often used interchangeably with Shrimp.

rPen a 1 from Brown shrimp (P. aztecus), is representative of other shrimp tropomyosin.

Recombinant allergens, which are genetically engineered isoforms resembling allergen molecules from known allergen extracts, have immunoglobulin E (IgE) antibody binding comparable to that of natural allergens and generally show excellent reactivity in in vitro and in vivo diagnostic tests. To date, many different recombinant allergens of pollens, molds, mites, bee venoms, foods and latex have been cloned, sequenced, and expressed. Recombinant allergens have a wide variety of uses, from the diagnosis and management of allergic patients to the development of immunotherapy to the standardisation of allergenic test products as tools in molecular allergology.

Recombinant Pen a 1 and natural Pen a 1 are structurally and immunologically identical.

At least 13 allergens are found in extracts of cooked Brown shrimp, and the 36-kDa muscle protein tropomyosin (Pen a 1) has been identified as a major Shrimp allergen. It is detected by sera of more than 80% of all subjects allergic to Shrimp and binds up to 75% of all Shrimp-specific IgE antibodies. Amino acid sequence identities with natural allergenic and non-allergenic tropomyosins ranged from 80% to 99% and 51% to 58%, respectively. Since Beef, Pork and Chicken are other tropomyosin-containing foods that are not very allergenic, tropomyosins can serve in investigations of the contribution of the varying structural properties of a protein to its allergenicity.

Recombinant allergens are particularly useful in addressing allergies that manifest wide cross-reactivity, such as allergies to crustaceans, Cockroaches and House dust mites.

Allergens from Penaeus aztecus listed by IUIS* Pen a 1 *International Union of Immunological Societies (www.allergen.org) Jan. 2008.

References
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